| Addison Smith - Hamilton Southeastern High School | 57.27 | 1:01.91 | ( 4.64 ) |
| Allison Fraze - Forest Park High School | 59.83 | 1:03.86 | ( 4.03 ) |
| Josefina Rastrelli - Warsaw Community High School | 56.93 | 1:00.58 | ( 3.65 ) |
| Julianna Crow - East Noble High School | 59.94 | 1:03.52 | ( 3.58 ) |
| Eden Wolfe - Indiana Storm Track Club | 58.59 | 1:01.86 | ( 3.27 ) |
| Ciara Kepner - Hamilton Southeastern High School | 56.91 | 1:00.03 | ( 3.12 ) |
| Emma Edwards - Edgewood High School | 55.36 | 58.19 | ( 2.83 ) |
| Kamiah Hunt - North Central High School (Indianapolis) | 58.87 | 1:01.70 | ( 2.83 ) |
| Kylee Regalado - Tri-West Hendricks High School | 57.57 | 1:00.24 | ( 2.67 ) |
| Iana Tecson - Evansville North High School | 1:00.12 | 1:02.18 | ( 2.06 ) |
| Kristina Petkova - Goshen High School | 56.40 | 58.39 | ( 1.99 ) |
| Athena Dixon - North Central High School (Indianapolis) | 58.72 | 1:00.69 | ( 1.97 ) |
| Karleigh Gillen - Angola High School | 59.85 | 1:01.78 | ( 1.93 ) |
| Leah Highley - Oak Hill High School | 58.36 | 1:00.07 | ( 1.71 ) |
| Addison Luers - Batesville High School | 1:00.17 | 1:01.80 | ( 1.63 ) |
| Jada Dale - Norwell High School | 58.75 | 1:00.20 | ( 1.45 ) |
| Aolani CANNON - Lawrence North High School | 1:00.47 | 1:01.86 | ( 1.39 ) |
| Savannah Nuetzel - Gary Steelers Track | 57.59 | 58.92 | ( 1.33 ) |
| Amiah Rhodes - Ben Davis High School | 59.75 | 1:01.05 | ( 1.30 ) |
| Igboamaka Odeluga - Munster High School | 59.03 | 1:00.32 | ( 1.29 ) |
| Scarlett Reksel - McCutcheon High School | 59.50 | 1:00.78 | ( 1.28 ) |
| Ellie Stewart - Plainfield High School | 58.74 | 59.96 | ( 1.22 ) |
| Breece Bass - Franklin Central High School | 56.36 | 57.53 | ( 1.17 ) |
| Addison Baxter - Columbia City High School | 1:00.31 | 1:01.47 | ( 1.16 ) |
| Emma Kincaid - Bremen High School | 59.01 | 1:00.11 | ( 1.10 ) |
| Lauren Klem - Franklin Community High School | 58.48 | 59.33 | ( 0.85 ) |
| Olivia Howell - Eastbrook High School | 59.08 | 59.90 | ( 0.82 ) |
| Ava Myers - Fort Wayne Carroll High School | 1:00.28 | 1:01.04 | ( 0.76 ) |
| Quinn Bird - Culver Academies | 57.02 | 57.77 | ( 0.75 ) |
| Gia Clay - Pike High School | 55.82 | 56.50 | ( 0.68 ) |
| Brailey Hoagland - Fountain Central High School | 59.91 | 1:00.57 | ( 0.66 ) |
| Morgan Manford - Lawrenceburg High School | 59.69 | 1:00.32 | ( 0.63 ) |
| Omema Anyanwu - Zionsville Community High School | 55.67 | 56.26 | ( 0.59 ) |
| Samantha Aselage - Fort Wayne Bishop Dwenger High School | 59.83 | 1:00.42 | ( 0.59 ) |
| Gabriela Grande Rosas - Indiana Storm Track Club | 57.87 | 58.39 | ( 0.52 ) |
| Henny Schminke - West Lafayette High School | 59.59 | 1:00.07 | ( 0.48 ) |
| Nicki Southerland - Delta High School | 57.59 | 58.05 | ( 0.46 ) |
| Jorie Irving - Valparaiso High School | 59.73 | 1:00.02 | ( 0.29 ) |
| Ava Olomajeye - Signature High School | 56.39 | 56.61 | ( 0.22 ) |
| Berkeley Williams - Plainfield High School | 1:00.33 | 1:00.51 | ( 0.18 ) |
| Elle Jo Johnson - North Posey High School | 58.84 | 58.91 | ( 0.07 ) |
| Addison Duncan - Bishop Chatard High School | 59.24 | 59.28 | ( 0.04 ) |
| Makena Cruse - Greenwood Community High School | 59.49 | 59.50 | ( 0.01 ) |